png output file of a matlab cbf analysis (MathWorks Inc)
Structured Review
Figure 4 D2). Note that there are lots of artefacts (appearing at the frequency of circa 10 Hz, blue in the ‘CBF raw’ and ‘CBF masked’ plots) visible on the mean power spectrum, CBF histogram and CBF heatmaps. (D) Remaining drifts in an aligned recording file. Images of the first (D1), middle (D2) and last (D3) frame of the 30s recording file. Each black arrow indicates an identical pixel site on the image. Note the loss of sharpness of the image at the arrow locations reflecting z drift. (E) Example of an unsuccessful fluorescent particle injection. The yellow arrow marks accumulated fluorescent particle in the brain parenchyma. Scale bar: 50 μm. " width="250" height="auto" />Png Output File Of A Matlab Cbf Analysis, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images
1) Product Images from "Measurement of ciliary beating and fluid flow in the zebrafish adult telencephalon"
Article Title: Measurement of ciliary beating and fluid flow in the zebrafish adult telencephalon
Journal: STAR Protocols
doi: 10.1016/j.xpro.2022.101542
Figure 4 D2). Note that there are lots of artefacts (appearing at the frequency of circa 10 Hz, blue in the ‘CBF raw’ and ‘CBF masked’ plots) visible on the mean power spectrum, CBF histogram and CBF heatmaps. (D) Remaining drifts in an aligned recording file. Images of the first (D1), middle (D2) and last (D3) frame of the 30s recording file. Each black arrow indicates an identical pixel site on the image. Note the loss of sharpness of the image at the arrow locations reflecting z drift. (E) Example of an unsuccessful fluorescent particle injection. The yellow arrow marks accumulated fluorescent particle in the brain parenchyma. Scale bar: 50 μm. " title="... (C) The PNG output file of a MATLAB CBF analysis on a non-aligned recording file with some ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Examples of good and bad brain explant and analysis (A and B) Good (B) and bad (A) examples of the adult brain explant. Dotted circles are the areas that experimenters should be careful. Small letters mark each dotted circle that show bad (a)-c)) and good examples (d)-f)). Scale bar: 50 μm. (C) The PNG output file of a MATLAB CBF analysis on a non-aligned recording file with some drift. Refer to the MATLAB CBF analysis from the aligned recording file (
Techniques Used: Injection
Figure Legend Snippet: Data analysis for ciliary beating images using a MATLAB script (A) The diagrams show the principle underlying the analysis for ciliary beating using the provided MATLAB scripts. (B) The interface of MATLAB. (C) The partial screenshot of “step 1” (C1) and “step 3” (C2) in the MATLAB script. The numbers with red lines can be modified to reflect the experimental or analyzing conditions. (D) Expected outcome of data analysis. (D1) Diagram of the brain explant (dorsal view). (D2) The PNG file output after analysis. The file contains the raw image, mean power spectrum, histogram of ciliary beating frequency (CBF) and color-coded raw/masked CBF. Red dotted circles with red arrows indicate difference between the results by the MATLAB script and FreQ plugin. Abbreviation: (D1) anterior (A), posterior (P), left (L) and right (R). (D2) Peak Frequency (Peak Freq) and Hertz (Hz). Scale bars are 50 μm.
Techniques Used: Modification
Figure Legend Snippet: Comparison of the data analysis for ciliary beating from the MATLAB script and FreQ plugin (A and B) Color-coded masked CBF plots from the MATLAB script (A) and the FreQ plugin (B). (C and D) The differences between the MATLAB and FreQ outputs are shown as a heat map of masked CBF (C) and histogram (D). Scale bars are 50 μm.
Techniques Used:
Figure Legend Snippet: Data analysis for ciliary beating using the FreQ plugin (A) The screenshots show various setup conditions on the parameters of the analysis in the FreQ plugin. (B) Expected outcome of the analysis. (B1) Diagram of the brain explant (dorsal view). (B2) The output PNG files after analysis. The files are color-coded raw/masked CBF, power spectrum of primary/secondary peak frequency, histogram of ciliary beating frequency (CBF). Red dotted circles with red arrows indicate difference between the results by the MATLAB script and FreQ plugin.
Techniques Used:
Figure Legend Snippet:
Techniques Used: Recombinant, Software, Microscopy, Imaging